Chromatin proteins in flowering

Research Project Dorota Komar, Instituto Nacional de Investigaciones Agrarias

The timing of flowering is crucial for the reproductive success of plants. In addition, flowering time directly impacts crop yield, making it a developmental process with considerable biotechnological potential. One of the main interests of our group is to understand the molecular mechanisms involved in the regulation of flowering and, particularly, in unveiling the pivotal role that chromatin remodeling plays in the control of gene expression patterns controlling the floral transition. The aim of this project is to further understand the role of the chromatin factor EBS in the silencing of flowering genes in Arabidopsis thaliana. For that, the fellow will use biochemical tools to identify effector proteins mediating the role of EBS in gene repression. The function of EBS will be analyzed by comparing the genome-wide binding sites of this protein (ChIP-seq) with transcriptomic profiles (RNA-seq) in leaves and vegetative meristems. The expression of master genes of flowering, and the distribution of histone marks in their genomic regions will be analyzed, comparing wild type plants with ebs mutants. The data obtained will be used to construct predictive models. To achieve these goals we will collaborate with partners UvA, WUR, IPG-PAS, BIOMOL and DIAG.