Research Project Johan Zicola, Max Planck Institute for Plant Breeding Research
The aim of the research project is to identify and characterize novel regulatory regions in candidate genes that regulate flowering in maize and Arabidopsis. To do so, Johan will use existing knowledge about chromatin modifications that are predominant at distal enhancers and profile these modifications during the reproductive phase shift in maize leaves. Chromatin modification patterns will be compared to transcriptome profiles generated by RNA-seq. To elucidate the molecular mechanism of enhancer-promoter interactions, he will establish the INTACT system for cell type-specific ChIP to generate histone modification profiles at the FT locus in A. thaliana phloem cells during absence and presence of FT expression. 3C experiments will be carried out to map dynamic physical interactions between regulatory regions. To achieve these goals he will collaborate with partners UvA, IPG-PAS, BIOMOL, DIAG and KG.