Research Project Wassim Lakhal, Diagenode
The aim of this project is the development of a robust and marketable ChIP protocol that is compatible with monocotyledonous and dicotyledonous plant species such as Zea mays, Glycine max, Oryza sativa, Solanum lycopersicum and Populus trichocarpa. In order to achieve this goal we collaborate with the University of Amsterdam, MPI for plant breeding in Cologne, Phytowelt in Cologne and the University of Duesseldorf, since these groups have proficient knowledge in setting up ChIP for various plant species and can provide us with the plant material. Next-generation sequencing will be applied in collaboration with the EpiTRAITS partners in order to generate ChIP-Seq profiles. Another aim of the project is the setup of cost-effective 5-mC screens on plant tissues followed by the generation of DNA methylation profiles by application of next-generation sequencing.